#!/usr/bin/env cwl-runner cwlVersion: v1.0 class: Workflow label: "umi duplex alignment fastq workflow" requirements: - class: SchemaDefRequirement types: - $import: ../types/sequence_data.yml - class: SubworkflowFeatureRequirement - class: ScatterFeatureRequirement inputs: sequence: type: ../types/sequence_data.yml#sequence_data[] label: "sequence: sequencing data and readgroup information" doc: | sequence represents the sequencing data as either FASTQs or BAMs with accompanying readgroup information. Note that in the @RG field ID and SM are required for FASTQs. For BAMs, this pipeline assumes that the RG information is already in the header. sample_name: type: string read_structure: type: string[] reference: type: - string - File secondaryFiles: [.fai, ^.dict, .amb, .ann, .bwt, .pac, .sa] target_intervals: type: File? outputs: aligned_bam: type: File secondaryFiles: [^.bai] outputSource: alignment_workflow/aligned_bam adapter_histogram: type: File[] outputSource: alignment_workflow/adapter_histogram duplex_seq_metrics: type: File[] outputSource: alignment_workflow/duplex_seq_metrics steps: sequence_to_bam: scatter: [sequence] scatterMethod: dotproduct run: ../tools/sequence_to_bam.cwl in: sequence: sequence out: [bam] alignment_workflow: run: ../subworkflows/duplex_alignment.cwl in: bam: sequence_to_bam/bam sample_name: sample_name read_structure: read_structure reference: reference target_intervals: target_intervals out: [aligned_bam, adapter_histogram, duplex_seq_metrics]