#!/usr/bin/env cwl-runner

cwlVersion: v1.0
class: Workflow
label: "alignment for nonhuman with qc"
requirements:
    - class: SchemaDefRequirement
      types:
          - $import: ../types/labelled_file.yml
          - $import: ../types/sequence_data.yml
          - $import: ../types/trimming_options.yml
    - class: SubworkflowFeatureRequirement
    - class: StepInputExpressionRequirement
inputs:
    reference:
        type:
            - string
            - File
        secondaryFiles: [.fai, ^.dict, .amb, .ann, .bwt, .pac, .sa]
    sequence:
        type: ../types/sequence_data.yml#sequence_data[]
        label: "sequence: sequencing data and readgroup information"
        doc: |
          sequence represents the sequencing data as either FASTQs or BAMs with accompanying
          readgroup information. Note that in the @RG field ID and SM are required.
    trimming:
        type:
            - ../types/trimming_options.yml#trimming_options
            - "null"
    final_name:
        type: string?
    per_base_intervals:
        type: ../types/labelled_file.yml#labelled_file[]
        default: []
    per_target_intervals:
        type: ../types/labelled_file.yml#labelled_file[]
        default: []
    summary_intervals:
        type: ../types/labelled_file.yml#labelled_file[]
        default: []
    picard_metric_accumulation_level:
        type: string
    minimum_mapping_quality:
        type: int?
    minimum_base_quality:
        type: int?
outputs:
    bam:
        type: File
        outputSource: alignment/final_bam
    mark_duplicates_metrics:
        type: File
        outputSource: alignment/mark_duplicates_metrics_file
    insert_size_metrics:
        type: File
        outputSource: qc/insert_size_metrics
    insert_size_histogram:
        type: File
        outputSource: qc/insert_size_histogram
    alignment_summary_metrics:
        type: File
        outputSource: qc/alignment_summary_metrics
    wgs_metrics:
        type: File
        outputSource: qc/wgs_metrics
    gc_bias_metrics:
        type: File
        outputSource: qc/gc_bias_metrics
    gc_bias_metrics_chart:
        type: File
        outputSource: qc/gc_bias_metrics_chart
    gc_bias_metrics_summary:
        type: File
        outputSource: qc/gc_bias_metrics_summary
    flagstats:
        type: File
        outputSource: qc/flagstats
    per_base_coverage_metrics:
        type: File[]
        outputSource: qc/per_base_coverage_metrics
    per_base_hs_metrics:
        type: File[]
        outputSource: qc/per_base_hs_metrics
    per_target_coverage_metrics:
        type: File[]
        outputSource: qc/per_target_coverage_metrics
    summary_hs_metrics:
        type: File[]
        outputSource: qc/summary_hs_metrics
    per_target_hs_metrics:
        type: File[]
        outputSource: qc/per_target_hs_metrics
    bamcoverage_bigwig:
        type: File
        outputSource: qc/bamcoverage_bigwig

steps:
    alignment:
        run: ../subworkflows/sequence_to_bqsr_nonhuman.cwl
        in:
            reference: reference
            unaligned: sequence
            trimming: trimming
            final_name: final_name
        out: [final_bam,mark_duplicates_metrics_file]
    qc:
        run: ../subworkflows/qc_wgs_nonhuman.cwl
        in:
            bam: alignment/final_bam
            reference: reference
            picard_metric_accumulation_level: picard_metric_accumulation_level
            minimum_mapping_quality: minimum_mapping_quality
            minimum_base_quality: minimum_base_quality
            per_base_intervals: per_base_intervals
            per_target_intervals: per_target_intervals
            summary_intervals: summary_intervals
        out: [insert_size_metrics,insert_size_histogram,alignment_summary_metrics,gc_bias_metrics,gc_bias_metrics_chart,gc_bias_metrics_summary,flagstats,per_base_coverage_metrics,per_base_hs_metrics,per_target_coverage_metrics,summary_hs_metrics,per_target_hs_metrics,bamcoverage_bigwig,wgs_metrics]