Term: pcr primers (pcr_primers)

PCR primers that were used to amplify the sequence of the targeted gene, locus or subfragment. This field should contain all the primers used for a single PCR reaction if multiple forward or reverse primers are present in a single PCR reaction. The primer sequence should be reported in uppercase letters

URI: MIXS:0000046

Applicable Checklists and Extensions

NOTE: does not include Combinations (of checklists and extensions) that use pcr_primers.

Name	Description	Checklist/Extension
MimarksS	Minimal Information about a Marker Specimen: survey	Checklist
Agriculture	A collection of terms appropriate when sequencing samples obtained in an agri...	Extension
MimarksC	Minimal Information about a Marker Specimen: specimen	Checklist

Properties

• Range: String
• Cardinality: 0..1

Examples

Value
FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT

Annotations

• Expected_value: FWD: forward primer sequence;REV:reverse primer sequence

LinkML Source

name: pcr_primers
annotations:
  Expected_value:
    tag: Expected_value
    value: 'FWD: forward primer sequence;REV:reverse primer sequence'
description: PCR primers that were used to amplify the sequence of the targeted gene,
  locus or subfragment. This field should contain all the primers used for a single
  PCR reaction if multiple forward or reverse primers are present in a single PCR
  reaction. The primer sequence should be reported in uppercase letters
title: pcr primers
examples:
- value: FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
keywords:
- pcr
string_serialization: FWD:{dna};REV:{dna}
slot_uri: MIXS:0000046
alias: pcr_primers
domain_of:
- MimarksC
- MimarksS
- Agriculture
range: string